![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1809012:Design
Designed by: Edward Dring Group: iGEM15_WPI-Worcester (2015-08-03)
TiAFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 658
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 91
Illegal AgeI site found at 220
Illegal AgeI site found at 250 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 511
Design Notes
Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.
Source
Taken from the fungus Typhula ishikariensis. Pubmed ID: 20030710